SURAT CHEMICAL TAG:
Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion-exchange chromatography, a type of column chromatography, for the separation and purification of proteins and nucleic acids.
DEAE into the H+ form you need to do a NaOH regeneration step, Water wash and then follow that with a HCl regeneration step, followed by the Water wash and equilibration to your buffer.
Cellulose is a molecule, consisting of hundreds – and sometimes even thousands – of carbon, hydrogen and oxygen atoms. Cellulose is the main substance in the walls of plant cells, helping plants to remain stiff and upright. Humans cannot digest cellulose, but it is important in the diet as fibre.
DEAE-Sephadex is a positively-charged slurry that will have electrostatic interactions with the negatively charged atoms, making them elute later than the positively-charged molecules in the interested sample. This is a separation technique used widely to discover specific proteins, or enzymes in the body.
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